Review



normal rat embryo fibroblasts  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    ATCC normal rat embryo fibroblasts
    Normal Rat Embryo Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/embryo+rat+fibroblasts/Rat2%3B+Embryo+Fibroblast%3B+Rat/us09486538-444-1-6
    Average 94 stars, based on 65 article reviews
    normal rat embryo fibroblasts - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Electron Microscopy:

    Article Title: Toward Origami-Inspired In Vitro Cardiac Tissue Models.
    Article Snippet: The enriched cardiac population (>70% cardiomyocytes) was seeded at a density of 6 × 104 cells/cm2 and cultured for 48 h, before starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v L-glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium).before starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v L-glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium). ... For pattern alignment studies, embryo rat fibroblasts (Rat2, Rattus norvegicus, ATCC-CRL-1764) were used and expanded in HG DMEM (Sigma-Aldrich, USA) supplemented with 10% (v/v) FBS (SigmaAldrich, USA) and 1% (v/v) penicillin/streptomycin (Sigma-Aldrich, USA).. 2.5.

    Article Title: Toward Origami-Inspired In Vitro Cardiac Tissue Models
    Article Snippet: The enriched cardiac population (>70% cardiomyocytes) was seeded at a density of 6 × 10 4 cells/cm 2 and cultured for 48 h, before starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v l -glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium).starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v l -glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium). ... For pattern alignment studies, embryo rat fibroblasts (Rat2, Rattus norvegicus , ATCC-CRL-1764) were used and expanded in HG DMEM (Sigma-Aldrich, USA) supplemented with 10% (v/v) FBS (Sigma-Aldrich, USA) and 1% (v/v) penicillin/streptomycin (Sigma-Aldrich, USA).

    Staining:

    Article Title: Toward Origami-Inspired In Vitro Cardiac Tissue Models.
    Article Snippet: The enriched cardiac population (>70% cardiomyocytes) was seeded at a density of 6 × 104 cells/cm2 and cultured for 48 h, before starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v L-glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium).before starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v L-glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium). ... For pattern alignment studies, embryo rat fibroblasts (Rat2, Rattus norvegicus, ATCC-CRL-1764) were used and expanded in HG DMEM (Sigma-Aldrich, USA) supplemented with 10% (v/v) FBS (SigmaAldrich, USA) and 1% (v/v) penicillin/streptomycin (Sigma-Aldrich, USA).. 2.5.

    Article Title: Toward Origami-Inspired In Vitro Cardiac Tissue Models
    Article Snippet: The enriched cardiac population (>70% cardiomyocytes) was seeded at a density of 6 × 10 4 cells/cm 2 and cultured for 48 h, before starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v l -glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium).starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v l -glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium). ... For pattern alignment studies, embryo rat fibroblasts (Rat2, Rattus norvegicus , ATCC-CRL-1764) were used and expanded in HG DMEM (Sigma-Aldrich, USA) supplemented with 10% (v/v) FBS (Sigma-Aldrich, USA) and 1% (v/v) penicillin/streptomycin (Sigma-Aldrich, USA).

    Immunofluorescence:

    Article Title: Toward Origami-Inspired In Vitro Cardiac Tissue Models.
    Article Snippet: The enriched cardiac population (>70% cardiomyocytes) was seeded at a density of 6 × 104 cells/cm2 and cultured for 48 h, before starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v L-glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium).before starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v L-glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium). ... For pattern alignment studies, embryo rat fibroblasts (Rat2, Rattus norvegicus, ATCC-CRL-1764) were used and expanded in HG DMEM (Sigma-Aldrich, USA) supplemented with 10% (v/v) FBS (SigmaAldrich, USA) and 1% (v/v) penicillin/streptomycin (Sigma-Aldrich, USA).. 2.5.

    Article Title: Toward Origami-Inspired In Vitro Cardiac Tissue Models
    Article Snippet: The enriched cardiac population (>70% cardiomyocytes) was seeded at a density of 6 × 10 4 cells/cm 2 and cultured for 48 h, before starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v l -glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium).starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v l -glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium). ... For pattern alignment studies, embryo rat fibroblasts (Rat2, Rattus norvegicus , ATCC-CRL-1764) were used and expanded in HG DMEM (Sigma-Aldrich, USA) supplemented with 10% (v/v) FBS (Sigma-Aldrich, USA) and 1% (v/v) penicillin/streptomycin (Sigma-Aldrich, USA).

    Cell Culture:

    Article Title: Toward Origami-Inspired In Vitro Cardiac Tissue Models.
    Article Snippet: The enriched cardiac population (>70% cardiomyocytes) was seeded at a density of 6 × 104 cells/cm2 and cultured for 48 h, before starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v L-glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium).before starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v L-glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium). ... For pattern alignment studies, embryo rat fibroblasts (Rat2, Rattus norvegicus, ATCC-CRL-1764) were used and expanded in HG DMEM (Sigma-Aldrich, USA) supplemented with 10% (v/v) FBS (SigmaAldrich, USA) and 1% (v/v) penicillin/streptomycin (Sigma-Aldrich, USA).. 2.5.

    Article Title: Toward Origami-Inspired In Vitro Cardiac Tissue Models
    Article Snippet: The enriched cardiac population (>70% cardiomyocytes) was seeded at a density of 6 × 10 4 cells/cm 2 and cultured for 48 h, before starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v l -glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium).starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v l -glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium). ... For pattern alignment studies, embryo rat fibroblasts (Rat2, Rattus norvegicus , ATCC-CRL-1764) were used and expanded in HG DMEM (Sigma-Aldrich, USA) supplemented with 10% (v/v) FBS (Sigma-Aldrich, USA) and 1% (v/v) penicillin/streptomycin (Sigma-Aldrich, USA).

    Functional Assay:

    Article Title: Toward Origami-Inspired In Vitro Cardiac Tissue Models.
    Article Snippet: The enriched cardiac population (>70% cardiomyocytes) was seeded at a density of 6 × 104 cells/cm2 and cultured for 48 h, before starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v L-glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium).before starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v L-glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium). ... For pattern alignment studies, embryo rat fibroblasts (Rat2, Rattus norvegicus, ATCC-CRL-1764) were used and expanded in HG DMEM (Sigma-Aldrich, USA) supplemented with 10% (v/v) FBS (SigmaAldrich, USA) and 1% (v/v) penicillin/streptomycin (Sigma-Aldrich, USA).. 2.5.

    Article Title: Toward Origami-Inspired In Vitro Cardiac Tissue Models
    Article Snippet: The enriched cardiac population (>70% cardiomyocytes) was seeded at a density of 6 × 10 4 cells/cm 2 and cultured for 48 h, before starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v l -glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium).starting the experiments, in high glucose (HG) Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, USA), supplemented with 1% v/v HEPES buffer (Sigma-Aldrich, USA), 1% v/v penicillin/streptomycin (Sigma-Aldrich, USA), 1% v/v l -glutamine (Sigma-Aldrich, USA), and 10% v/v fetal bovine serum (FBS, Sigma-Aldrich, USA) (seeding medium). ... For pattern alignment studies, embryo rat fibroblasts (Rat2, Rattus norvegicus , ATCC-CRL-1764) were used and expanded in HG DMEM (Sigma-Aldrich, USA) supplemented with 10% (v/v) FBS (Sigma-Aldrich, USA) and 1% (v/v) penicillin/streptomycin (Sigma-Aldrich, USA).



    Similar Products

    normal rat embryo fibroblasts  (ATCC)
    94
    ATCC normal rat embryo fibroblasts
    Normal Rat Embryo Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/embryo+rat+fibroblasts/Rat2%3B+Embryo+Fibroblast%3B+Rat/us09486538-444-1-6
    Average 94 stars, based on 1 article reviews
    normal rat embryo fibroblasts - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    embryo rat fibroblasts  (ATCC)
    95
    ATCC embryo rat fibroblasts
    Figure 2. Cell alignment on different patterns. (A) Scanning electron microscopy images of PDMS molds and rat fibroblast (ATCC CRL-1764) alignment on patterned substrates. PDMS replica from custom-made SU8 microfabricated molds. (B) Rhodamine-phalloidin staining of <t>rat2</t> <t>fibroblasts</t> grown on cross-linked 10% w/v gelatin hydrogels patterned with the PDMS molds showed good alignment on 25 and 50 μm wide structures (CLSM image of the bottom surface). (C) Directionality histograms of the actin cytoskeleton of cells growing on the 25 and 50 μm structures shown in panel (B). Scale bars: 50 μm.
    Embryo Rat Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/embryo+rat+fibroblasts/Rat2/pm39978771-76-4-10
    Average 95 stars, based on 1 article reviews
    embryo rat fibroblasts - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    rat embryo fibroblasts ref  (Johns Hopkins HealthCare)
    90
    Johns Hopkins HealthCare rat embryo fibroblasts ref
    Figure 2. Cell alignment on different patterns. (A) Scanning electron microscopy images of PDMS molds and rat fibroblast (ATCC CRL-1764) alignment on patterned substrates. PDMS replica from custom-made SU8 microfabricated molds. (B) Rhodamine-phalloidin staining of <t>rat2</t> <t>fibroblasts</t> grown on cross-linked 10% w/v gelatin hydrogels patterned with the PDMS molds showed good alignment on 25 and 50 μm wide structures (CLSM image of the bottom surface). (C) Directionality histograms of the actin cytoskeleton of cells growing on the 25 and 50 μm structures shown in panel (B). Scale bars: 50 μm.
    Rat Embryo Fibroblasts Ref, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/embryo+rat+fibroblasts/mouse+embryonic+fibroblasts/pmc10921015-51-0-8
    Average 90 stars, based on 1 article reviews
    rat embryo fibroblasts ref - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    rat primary embryo fibroblasts  (Dawley Inc)
    90
    Dawley Inc rat primary embryo fibroblasts
    Figure 2. Cell alignment on different patterns. (A) Scanning electron microscopy images of PDMS molds and rat fibroblast (ATCC CRL-1764) alignment on patterned substrates. PDMS replica from custom-made SU8 microfabricated molds. (B) Rhodamine-phalloidin staining of <t>rat2</t> <t>fibroblasts</t> grown on cross-linked 10% w/v gelatin hydrogels patterned with the PDMS molds showed good alignment on 25 and 50 μm wide structures (CLSM image of the bottom surface). (C) Directionality histograms of the actin cytoskeleton of cells growing on the 25 and 50 μm structures shown in panel (B). Scale bars: 50 μm.
    Rat Primary Embryo Fibroblasts, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/embryo+rat+fibroblasts/embryonic+fibroblasts/pm30712435-46-45-43
    Average 90 stars, based on 1 article reviews
    rat primary embryo fibroblasts - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    rat embryo fibroblast ref cells  (ATCC)
    95
    ATCC rat embryo fibroblast ref cells
    Figure 2. Cell alignment on different patterns. (A) Scanning electron microscopy images of PDMS molds and rat fibroblast (ATCC CRL-1764) alignment on patterned substrates. PDMS replica from custom-made SU8 microfabricated molds. (B) Rhodamine-phalloidin staining of <t>rat2</t> <t>fibroblasts</t> grown on cross-linked 10% w/v gelatin hydrogels patterned with the PDMS molds showed good alignment on 25 and 50 μm wide structures (CLSM image of the bottom surface). (C) Directionality histograms of the actin cytoskeleton of cells growing on the 25 and 50 μm structures shown in panel (B). Scale bars: 50 μm.
    Rat Embryo Fibroblast Ref Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/embryo+rat+fibroblasts/Rat2/us10555961-235-0-8
    Average 95 stars, based on 1 article reviews
    rat embryo fibroblast ref cells - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    rat primary embryo fibroblasts (ref)  (Dawley Inc)
    90
    Dawley Inc rat primary embryo fibroblasts (ref)
    Figure 2. Cell alignment on different patterns. (A) Scanning electron microscopy images of PDMS molds and rat fibroblast (ATCC CRL-1764) alignment on patterned substrates. PDMS replica from custom-made SU8 microfabricated molds. (B) Rhodamine-phalloidin staining of <t>rat2</t> <t>fibroblasts</t> grown on cross-linked 10% w/v gelatin hydrogels patterned with the PDMS molds showed good alignment on 25 and 50 μm wide structures (CLSM image of the bottom surface). (C) Directionality histograms of the actin cytoskeleton of cells growing on the 25 and 50 μm structures shown in panel (B). Scale bars: 50 μm.
    Rat Primary Embryo Fibroblasts (Ref), supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/embryo+rat+fibroblasts/embryonic+fibroblasts/pmc06464594-98-37-35
    Average 90 stars, based on 1 article reviews
    rat primary embryo fibroblasts (ref) - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    rat primary embryo fibroblasts ref  (Dawley Inc)
    90
    Dawley Inc rat primary embryo fibroblasts ref
    Figure 2. Cell alignment on different patterns. (A) Scanning electron microscopy images of PDMS molds and rat fibroblast (ATCC CRL-1764) alignment on patterned substrates. PDMS replica from custom-made SU8 microfabricated molds. (B) Rhodamine-phalloidin staining of <t>rat2</t> <t>fibroblasts</t> grown on cross-linked 10% w/v gelatin hydrogels patterned with the PDMS molds showed good alignment on 25 and 50 μm wide structures (CLSM image of the bottom surface). (C) Directionality histograms of the actin cytoskeleton of cells growing on the 25 and 50 μm structures shown in panel (B). Scale bars: 50 μm.
    Rat Primary Embryo Fibroblasts Ref, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/embryo+rat+fibroblasts/embryonic+fibroblasts/pmc06464594-64-44-45
    Average 90 stars, based on 1 article reviews
    rat primary embryo fibroblasts ref - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 2. Cell alignment on different patterns. (A) Scanning electron microscopy images of PDMS molds and rat fibroblast (ATCC CRL-1764) alignment on patterned substrates. PDMS replica from custom-made SU8 microfabricated molds. (B) Rhodamine-phalloidin staining of rat2 fibroblasts grown on cross-linked 10% w/v gelatin hydrogels patterned with the PDMS molds showed good alignment on 25 and 50 μm wide structures (CLSM image of the bottom surface). (C) Directionality histograms of the actin cytoskeleton of cells growing on the 25 and 50 μm structures shown in panel (B). Scale bars: 50 μm.

    Journal: ACS biomaterials science & engineering

    Article Title: Toward Origami-Inspired In Vitro Cardiac Tissue Models.

    doi: 10.1021/acsbiomaterials.4c01594

    Figure Lengend Snippet: Figure 2. Cell alignment on different patterns. (A) Scanning electron microscopy images of PDMS molds and rat fibroblast (ATCC CRL-1764) alignment on patterned substrates. PDMS replica from custom-made SU8 microfabricated molds. (B) Rhodamine-phalloidin staining of rat2 fibroblasts grown on cross-linked 10% w/v gelatin hydrogels patterned with the PDMS molds showed good alignment on 25 and 50 μm wide structures (CLSM image of the bottom surface). (C) Directionality histograms of the actin cytoskeleton of cells growing on the 25 and 50 μm structures shown in panel (B). Scale bars: 50 μm.

    Article Snippet: For pattern alignment studies, embryo rat fibroblasts (Rat2, Rattus norvegicus, ATCC-CRL-1764) were used and expanded in HG DMEM (Sigma-Aldrich, USA) supplemented with 10% (v/v) FBS (SigmaAldrich, USA) and 1% (v/v) penicillin/streptomycin (Sigma-Aldrich, USA).

    Techniques: Electron Microscopy, Staining